| SCIENTIFIC ACTIVITY |
| Number of grants 2004-2006: 11 |
| Areas of scientific activity: Structure, dynamics and specific
intermolecular interactions of proteins, nucleic acids
and their components. Influence of physic-chemical properties of mutagenic, antivirial
and antitumor agents on the biological activity. Physical basis of molecular mechanisms
of gene expression and regulation: initiation of protein biosynthesis, electron and
proton transfer, enzymatic phosphorylation and phosphorolysis, protein folding. |
Experimental techniques: molecular spectroscopy (UV-VIS,
fluorescence emission, stopped-flow, NMR, CD),
X-ray diffraction, molecular photophysics, electrochemistry, chemistry, enzymology, genetic
engineering.
Theoretical methods: classical, Brownian, and quantum molecular dynamics, molecular
modeling, quantum chemistry. |
| Equipment: Time-resolved spectrofluorimeter System 5000 IBH
Consultants Ltd, stopped-flow spectrometer SX.18MV Applied Photophysics Ltd., spectrofluorimeters:
LS50B Perkin Elmer, FluoroMax Spex, Shimadzu 500, ITC calorimeter OMEGA MicroCal Inc., cenrifuge Avanti J-20I Beckman-Coulter,
7 UV VIS and 2 IR spectrophotometers, HPLC Waters, FPLC Amersham-Pharmacia, CV-37
Voltammograph Bioanalytical Systems, 3 Silicon Graphics workstations and ca. 30 PC computers;
access to NMR spectrometer Varian UNITYplus 500 MHz, CD spectrometer Avis, MS spectrometer
Q-Tof2 Micromass and Cray computer. |
| Research groups: |
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Studies of the macromolecular structure, dynamics and function in solution |
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Purine nucleoside phosphorylases (PNP): three-dimensional structure and
molecular mechanism of catalysis
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Role of 5' mRNA and 5' U snRNA cap structures in regulation of gene expression |
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Mechanism of action of selected biomacromolecules and
their constituents, with the aid of absorption and emission (fluorescence, phosphorescence)
spectroscopy, and kinetic methods, including time-resolved techniques |
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Translation initiation and intracellular transport: biophysics of
protein and RNA interactions |
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Research
