Laboratory of Biopolymers

Physics as the basis for new technologies - development of modern research infrastructure at the Faculty of Physics of the University of Warsaw POIG.02.01.00-14-122/09
co-financed by European Union from ERDF within Innovation Economy Operational Programme 2007-2013
Priority 2. Infrastructure B+R,
Funding 39 814 000 PLN
Duration January 1, 2010 to December 31, 2012

Project description

The project is aimed at significant extension and development of the experimental possibilities of Division of Biophysics by creation of a Laboratory of Biopolymers equipped with modern and unique scientific instruments. Launching of the project enables complex and multidisciplinary approach to investigations of proteins and ribonucleic acids (RNA), directed to their activity within the molecular complexes and subcellular nanostructures. The research is based on wide collaboration of physicists, chemists and biologists, and range from providing the suitable objects by means of chemical syntheses (RNA fragments, enzyme inhibitors) and genetic engineering (protein factors and enzymes), their structural and dynamic characterization by means of biophysical methods, and their function in biological in vitro and in vivo systems, taking into account practical aspects of the results in rational drug design. Expensive and worldwide high throughput investigations in genomics and proteomics should be supplemented by dynamic, thermodynamic, and energetic aspects, in terms of molecular biophysics as well as multidisciplinary approach including chemistry, physics and biology. Exploiting of the new instrumentation in the Laboratory of Biopolymers will follow that strategy. Close collaboration within the Project between the Laboratory of Biopolymers and Laboratory of Modelling Large Biomolecular Complexes and Nanostructures guarantees a support from modern bioinformatics and professional computer packages for classical and quantum calculations and simulations in the experimental work. The Laboratory of Biopolymers is open for a worldwide collaboration with other scientific laboratories.

List of instruments

  1. X-ray diffractometer SuperNova A (Cu) Diffraction System Oxford Diffraction
  2. Multifunctional system of emission spectroscopy FCS-FLIM
  3. Spectrophotometer CD CHIRASCAN Applied Photophysics
  4. Micro calorimeter ITC-200 MicroCal
  5. Mass spectrometer API 3200 AB Sciex
  6. Synthesizer DNA/RNA ÄKTA OLIGOPILOTplus Donserv
  7. Thermocycler "Real Time" PCR CFX 96 Bio-Rad
  8. Automatic system of electrophoresis EXPERION Bio-Rad
  9. Liquid chromatograph HPLC AGILENT 1200
  10. Preparative centrifuge Avanti J-26 XPI Beckman Coulter
  11. Autoclav LABOKLAV 135 V Labo Baza