Prof. Dr hab. Jan Antosiewicz

Division of Biophysics
Institute of Experimental Physics
University of Warsaw
Pasteura 5, 02-093 Warsaw, Poland

Phone: +48 22 5532340

E-mail: Jan.Antosiewicz@fuw.edu.pl

Current research interests

  • Application and development of stopped-flow, laser flash photolysis, brownian dynamics and molecular dynamics methods to study electrostatic and hydrodynamic effects in kinetics of molecular association and molecular dynamics.

  • Investigation of protein-ligand association by stacionary and transient near and far UV CD spectroscopy, in particular to proteins binding analogs of 5'-end of m-RNA.

           Past research interests:

  • Constant-pH molecular dynamics and molecular electrostatics simulations of proteins and protein-ligand systems.

  • Investigation of kinetics of protein-ligand association by Brownian dynamics simulations.

  • Investigation of multiple protonation equilibria in proteins using Poisson-Boltzmann model of their aqueous solutions.

  • Analysis of macromolecular structures and their dynamics in solution by quantitative electro-optics.

  • Investigation of biopolymer dynamics by T-jump spectroscopy.

  • Investigation of hydration of biomolecules by ultrasonic velocimetry of their aqueous-alcoholic solutions.

Professional experience

1978 - 1986 Department of Biophysics, Warsaw University, Warsaw, Poland; Research Associate
1986 - 1988 Max Planck Institut für biophysikalische Chemie, Goettingen, Germany; Postdoctoral Research Associate.
1988 - 1992 Department of Biophysics, Warsaw University, Warsaw, Poland; Adjunct.
1992 - 1995 Institute for Molecular Design, University of Houston, Houston, Texas, USA; Postdoctoral Research Associate; and Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, USA; Visiting Assistant Research Chemist.
1995 - 2001 Department of Biophysics, Warsaw University, Warsaw, Poland; Adjunct.
2002 - Department of Biophysics, Warsaw University, Warsaw, Poland; Professor

Publications (2014-2018)

  1. M. Dlugosz, J. M. Antosiewicz, Evaluation of proteins' rotational diffusion coefficients from simulations of their free Brownian motion in volume-occupied environments, J. Chem. Theory Comp., 10:481-491 (2014)
  2. M. Dlugosz, J. M. Antosiewicz, Transient effects of molecular crowding on translational diffusion of anisotropic molecules, J. Chem. Theory Comp., 10:2583-2590 (2014)
  3. J. Wierzchowski, J. M. Antosiewicz, D. Shugar, 8-Azapurines as isosteric purine fluorescent probes for nucleic acid and enzymatic research, Molecular BioSystems, 10:2756-2774 (2014)
  4. M. Dlugosz, J. M. Antosiewicz, Toward accurate modeling of hydrodynamic effects on the translational and rotational dynamics of biomolecules in many-body systems, J. Phys. Chem. B, 119:8425-8439 (2015)
  5. J. M. Antosiewicz, D. Shugar, UV-Vis spectroscopy of tyrosine side-groups in studies of protein structure. Part 1: Basic principles and properties of tyrosine chromophore, Biophysical Reviews, 8:151–161 (2016)
  6. J. M. Antosiewicz, D. Shugar, UV-Vis spectroscopy of tyrosine side-groups in studies of protein structure. Part 2: Selected applications, Biophysical Reviews, 8:163–177 (2016)
  7. M. Dlugosz, J. M. Antosiewicz, Effects of Spatially-Dependent Mobilities of the Kinetics of the Diffusion-Controlled Association Derived from the First Passage Time Approach, J. Phys. Chem. B, 120:7114–7127 (2016)
  8. J. M. Antosiewicz, K. Kaminski and M. Dlugosz, Hydrodynamic Steering in Protein Association Revisited: Surprisingly Minuscule Effects of Considerable Torques, J. Phys. Chem. B, 121:8475-8491 (2017)
  9. J. M. Antosiewicz and M. Dlugosz, Does Ionic Screening Lower Activation Barriers for Conformational Transitions in Proteins?, J. Phys. Chem. B, 122:11817-11826 (2018)