Prof. Dr hab. Jan Antosiewicz

Division of Biophysics
Institute of Experimental Physics
University of Warsaw
Zwirki i Wigury 93, 02-089 Warsaw, Poland

Phone: +48 22 5540786

E-mail: jantosi@biogeo.uw.edu.pl

Current research interests

  • Application and development of stopped-flow, laser flash photolysis, brownian dynamics and molecular dynamics methods to study electrostatic and hydrodynamic effects in kinetics of molecular association and molecular dynamics.

  • Investigation of protein-ligand association by stacionary and transient near and far UV CD spectroscopy, in particular to proteins binding analogs of 5'-end of m-RNA.

           Past research interests:

  • Constant-pH molecular dynamics and molecular electrostatics simulations of proteins and protein-ligand systems.

  • Investigation of kinetics of protein-ligand association by Brownian dynamics simulations.

  • Investigation of multiple protonation equilibria in proteins using Poisson-Boltzmann model of their aqueous solutions.

  • Analysis of macromolecular structures and their dynamics in solution by quantitative electro-optics.

  • Investigation of biopolymer dynamics by T-jump spectroscopy.

  • Investigation of hydration of biomolecules by ultrasonic velocimetry of their aqueous-alcoholic solutions.

Professional experience

1978 - 1986 Department of Biophysics, Warsaw University, Warsaw, Poland; Research Associate
1986 - 1988 Max Planck Institut für biophysikalische Chemie, Goettingen, Germany; Postdoctoral Research Associate.
1988 - 1992 Department of Biophysics, Warsaw University, Warsaw, Poland; Adjunct.
1992 - 1995 Institute for Molecular Design, University of Houston, Houston, Texas, USA; Postdoctoral Research Associate; and Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, USA; Visiting Assistant Research Chemist.
1995 - 2001 Department of Biophysics, Warsaw University, Warsaw, Poland; Adjunct.
2002 - Department of Biophysics, Warsaw University, Warsaw, Poland; Professor

Publications (2012-2016)

  1. M. Dlugosz, J. M. Antosiewicz, P. Zielinski, J. Trylska, Contributions of Far-Field Hydrodynamic Interactions to the Kinetics of Electrostatically Driven Molecular Association, J. Phys. Chem. B, 116:5437-5447 (2012)
  2. M. Dlugosz, J. M. Antosiewicz, Anisotropic diffusion effects on the barnase-barstar encounter kinetics, J. Chem. Theory Comp., 9:1667-1677 (2013)
  3. M. Dlugosz, J. M. Antosiewicz, Hydrodynanamic effects on the relative rotational velocity of associating proteins, J. Phys. Chem. B, 117:6167-6174 (2013)
  4. Z. Gasik, D. Shugar, J. M. Antosiewicz, Resolving Differences in Substrate Specificities between Human and Parasite Phosphoribosyltransferases via Analysis of Functional Groups of Substrates and Receptors, Current Pharmaceutical Design, 19:4226-4240 (2013)
  5. M. Dlugosz, J. M. Antosiewicz, Evaluation of proteins' rotational diffusion coefficients from simulations of their free Brownian motion in volume-occupied environments, J. Chem. Theory Comp., 10:481-491 (2014)
  6. M. Dlugosz, J. M. Antosiewicz, Transient effects of molecular crowding on translational diffusion of anisotropic molecules, J. Chem. Theory Comp., 10:2583-2590 (2014)
  7. J. Wierzchowski, J. M. Antosiewicz, D. Shugar, 8-Azapurines as isosteric purine fluorescent probes for nucleic acid and enzymatic research, Molecular BioSystems, 10:2756-2774 (2014)
  8. M. Dlugosz, J. M. Antosiewicz, Toward accurate modeling of hydrodynamic effects on the translational and rotational dynamics of biomolecules in many-body systems, J. Phys. Chem. B, 119:8425-8439 (2015)
  9. J. M. Antosiewicz, D. Shugar, UV-Vis spectroscopy of tyrosine side-groups in studies of protein structure. Part 1: Basic principles and properties of tyrosine chromophore, Biophysical Reviews, 8:151–161 (2016)
  10. J. M. Antosiewicz, D. Shugar, UV-Vis spectroscopy of tyrosine side-groups in studies of protein structure. Part 2: Selected applications, Biophysical Reviews, 8:163–177 (2016)
  11. M. Dlugosz, J. M. Antosiewicz, Effects of Spatially-Dependent Mobilities of the Kinetics of the Diffusion-Controlled Association Derived from the First Passage Time Approach, J. Phys. Chem. B, 120:7114–7127 (2016)