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Warsaw University Faculty of Physics
WERSJA POLSKA  Research    People    Education
Name: Prof.dr hab. Jan Antosiewicz
Address: Department of Biophysics, Institute of Experimental Physics, Warsaw University, ¯wirki i Wigury 93, 02-089 Warsaw, Poland
Phone: +48 22 5540786
E-mail: jantosi@biogeo.uw.edu.pl

Current research interests:

  1. Application and development of stopped-flow, laser flash photolysis, brownian dynamics and molecular dynamics methods to study electrostatic and hydrodynamic effects in kinetics of molecular association and molecular dynamics.
  2. Constant-pH molecular dynamics and molecular electrostatics simulations of proteins and protein-ligand systems.
  3. Analysis of macromolecular structures and their dynamics in solution by quantitative electro-optics.
  4. Investigation of protein-ligand association by stacionary and transient near and far UV CD spectroscopy, in particular to proteins binding analogs of 5'-end of m-RNA.
Professional experience:
1978 - 1986 Department of Biophysics, Warsaw University, Warsaw, Poland; Research Associate.
1986 - 1988 Max Planck Institut für biophysikalische Chemie, Goettingen, Germany; Postdoctoral Research Associate.
1988 - 1992 Department of Biophysics, Warsaw University, Warsaw, Poland; Adjunct.
1992 - 1995 Institute for Molecular Design, University of Houston, Houston, Texas, USA; Postdoctoral Research Associate; and Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, USA; Visiting Assistant Research Chemist.
1995 - 2001 Department of Biophysics, Warsaw University, Warsaw, Poland; Adjunct.
2002 - Department of Biophysics, Warsaw University, Warsaw, Poland; Professor.
Selected papers 2007-2011:
  1. E. Blachut-Okrasinska, E. Bojarska, J. Stepinski, and J. M. Antosiewicz, Kinetics of binding the mRNA cap analogues to the the translation initiation factor eIF4E under second-order reaction conditions, Biophys. Chem., 129:289-297 (2007)
  2. E. Blachut-Okrasinska, and J. M. Antosiewicz, Brownian dynamics simulations of binding mRNA cap analogues to eIF4E protein, J. Phys. Chem. B. 111: 13107-13115 (2007)
  3. J. M. Antosiewicz, Protonation free energy levels in complex molecular systems, Biopolymers 89: 262-269 (2008)
  4. Z. Pilat, J. M. Antosiewicz, Multiple protonation equilibria in electrostatics of protein-protein binding, J. Phys. Chem. B, 112: 15074-15085 (2008)
  5. O. Szklarczyk, J. Zuberek and J. M. Antosiewicz, Poisson-Boltzmann model analysis of binding mRNA cap analogues to the translation initiation factor eIF4E,Biophys. Chem., 140:16-23 (2009)
  6. J. M. Antosiewicz and D. Porschke, Effects of hydrodynamic coupling on electro-optical transients, J. Phys. Chem. B, 113:13988-13992 (2009)
  7. Z. Pilat, J. M. Antosiewicz, pKas of ionizable groups and energetics of protein conformational transitions, J. Phys. Chem. B, 114:1393-1406 (2010)
  8. J. M. Antosiewicz, D. Shugar, Poisson-Boltzmann continuum-solvation model: Applications to pH-dependent properties of biomolecules, Mol. BioSyst., 7:2923-2949 (2011)
Participation in research projects:
Studies of the macromolecular structure, dynamics and function in solution

Purine nucleoside phosphorylases (PNP): three-dimensional structure and molecular mechanism of catalysis

Translation initiation and intracellular transport: biophysics of protein and mRNA interactions

Role of 5' mRNA and 5' U snRNA cap structures in regulation of gene expression