Dr Elżbieta Bojarska

Research interests

1. the mechanisms of redox processes of purine derivatives (electrochemical, photochemical and enzymatic stydies)
2. kinetic of protein-ligand interactions (pH and ionic strength dependence)

Methods

1. stopped-flow spectroscopy
2. steady-state fluorescence
3. electrochemistry
4. photochemistry

Professional experience

1982 - 1987	phD studies in the Institute of Biochemistry and Biophysics PAN
1987 - 1993	technical assistance in the Department of Biophysics UW
1994 -	adiunkt in the Department of Biophysics UW
1991	post-doctoral stay (3 months) in the Department of Chemistry at the University of Turku (Finland)
1994	Individual Mobility Grant within TEMPUS programm (University Paris VII in France and University of Coimbra in Portugal), 4 months
1997 - 1998	2-months academic appointments in the Laboratory of Biorheology, Hydrodyn and Physicochemistry CNRC at the University Paris VII (POLONIUM project)
2001-2002	2-months academic appointments in the Laboratory of Molecular Photophysics CNRS at the University Paris-Sud in Orsay (POLONIUM project)

Selected publications

1. E. Bojarska, E. Darżynkiewicz, Z. Wieczorek, B. Czochralska, Electrochemical and spectroscopic study of redox transformation of the 7-methylguanine analog of coenzyme NAD+, Bioelectrochem. Bioenerg. 33 (1994) 181-190
2. E. Bojarska, K. Pawlicki, B. Czochralska, Photocatalytic reduction of nicotinamide coenzymes in the presence of titanium dioxide: The influence of aliphatic aminoacids, J. Photochem. Photobiol. A: Chemistry 108 (1997) 207-213
3. E. Bojarska, B. Czochralska, Electrooxidation of the antileukemic 2-chloro-2'-deoxy-adenosine and related compounds, J. Electroanal. Chem. 477 (1999) 89-96
4. E. Błachut-Okrasińska, E. Bojarska, A. Niedźwiecka, L. Chlebicka, E. Darżynkiewicz, R. Stolarski, J. Stępiński, J.M. Antosiewicz, Stopped-flow and Brownian dynamics studies of electrostatic effects in the kinetics of binding of 7-methyl-GpppG to the protein eIF4E, Eur. Biophys. J. 29 (2000) 487-498
5. E. Bojarska, E. Darżynkiewicz, Molekularne mechanizmy degradacji mRNA w komórkach eukariotycznych, Na pograniczu chemii i biologii, tom V (2002) 53-72, Wydawnictwo Naukowe UAM, Poznań
6. M. Długosz, E. Błachut-Okrasińska, E. Bojarska, E. Darżynkiewicz, J.M. Antosiewicz, Effects of pH on kinetics of binding of mRNA-cap analogs by translation initiation factor eIF4E, Eur. Biophys. J. 31 (2003) 606-616

Participation in research projects

Redox processes of halogenoderivatives of purine bases - laser photolysis, enzymatic kinetics (POLONIUM project 2001-2002)

Initiation of translation process - the mechanism of protein interactions with 5'-mRNA cap analogs

Effects of pH and ionic strength on the kinetic of eIF4E binding with 5'-mRNA cap analogs