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Warsaw University Faculty of Physics
WERSJA POLSKA  Research    People    Education
Name: Dr. Jacek Jemielity
Address: Department of Biophysics, Institute of Experimental Physics, Warsaw University, Zwirki i Wigury 93, 02-089 Warsaw, Poland
Phone: +48 22 5540774
or +48 22 5540788
E-mail: jacekj@biogeo.uw.edu.pl
Research interests: Synthesis of the modified analogs 5' end of mRNA and others biologically important nucleotides and nucleosides. Studies of the role mRNA cap in translation process. Inhibitory properties of the cap analogues in protein biosynthesis. Synthesis of a labeled compounds and their use in the studies of enzymatic reaction mechanisms.
Education and professional experience:
June 1997 M.Sc. - in Organic Chemistry, Faculty of Chemistry, Warsaw University, Poland.
January 2002 Ph.D. - in Organic Chemistry, Faculty of Chemistry, Warsaw University, Poland.
July 2002 Institute of Structural Biology, Grenoble, France (1 month). Inhibiton of protein biosynthesis with synthetic mRNA cap analogues.
Feb. 2002 - Sept. 2002 Assistant - Department of Biophysics, Institute of Experimental Physics, Warsaw University, Poland;
June - August 2003 University of Turku, Turku, Finland (3 months). Solid phase synthesis of capped oligonucleotides (mRNA cap analogues).
October 2002 - Adjunct - Department of Biophysics, Institute of Experimental Physics, Warsaw University, Poland;
Selected publications 1998-2008:
  1. Kowalska J, Lewdorowicz M, Zuberek J, Grudzien-Nogalska E, Bojarska E, Stepinski J, Rhoads RE, Darzynkiewicz E, Davis RE, Jemielity J.; Synthesis and Characterization of mRNA Cap Analogs Containing Phosphorothioate Substitutions that Bind Tightly to eIF4E and are Resistant to the Decapping Pyrophosphatase DcpS. RNA in press (2008).
  2. Deshmukh MV, Jones BN, Quang-Dang D-U, Flinders J, Floor SN, Kim C, Jemielity J, Kalek M, Darzynkiewicz E, Gross JD.; mRNA Decapping Is Promoted by an RNA-Binding Channel in Dcp2. Molec. Cell 29, 324-336 (2008).
  3. Grudzien-Nogalska E, Jemielity J, Kowalska J, Darzynkiewicz E, Rhoads RE.; Phosphorothioate cap analogs stabilize mRNA and increase translational efficiency in mammalian cells. RNA 13, 1745-1755 (2007).
  4. Kowalska J, Lewdorowicz M, Darzynkiewicz E, Jemielity J.; A simple and rapid synthesis of nucleotide analogues containing a phosphorothioate moiety at the terminal position of the phosphate chain. Tetrahedron Lett. 48, 5475-5479 (2007).
  5. Grudzien-Nogalska E, Stepinski J, Jemielity J, Zuberek J, Stolarski R, Rhoads RE, Darzynkiewicz E.; Synthesis of anti-reverse cap analogs (ARCAs) and their applications in mRNA translation and stability. Method Enzymol. 431, 203-227 (2007).
  6. Darzynkiewicz ZM, Bojarska E, Kowalska J, Lewdorowicz M, Jemielity J, Kalek M, Stepinski J, Davis RE, Darzynkiewicz E.; Interaction of human decapping scavenger with 5 ' mRNA cap analogues: structural requirements for catalytic activity. J Phys-Condens Mat. 19 art. no. 285217 (2007)
  7. Zuberek J, Kubacka D, Jablonowska A, Jemielity J, Stepinski J, Sonenberg N, Darzynkiewicz E.; Weak binding affinity of human 4EHP for mRNA cap analogs. RNA 13, 691-697 (2007).
  8. Kowalska J, Lewdorowicz M, Zuberek J, Bojarska E, Stepinski J, Stolarski R, Darzynkiewicz E, Jemielity J.; Assignment of the absolute configuration of P-chiral 5'mRNA cap analogues containing phosphorothioate moiety. Nucleos. Nucleot. Nucl. Acids 26, 1301-1305 (2007).
  9. Jemielity J, Stolarski R, Darzynkiewicz E.; Synthesis of H-3 and C-13 labeled mRNA cap dinucleotides-useful tools for NMR, biochemical, and biological studies. Nucleos. Nucleot. Nucl. Acids 26, 1315-1319 (2007).
  10. Lewdorowicz M, Jemielity J, Kierzek R, Shapira M, Stepinski J, Darzynkiewicz E.; Solid-supported synthesis of 5 '-mRNA cap-4 from trypanosomatids. . Nucleos. Nucleot. Nucl. Acids 26, 1329-1333 (2007).
  11. Wierzchowski J, Pietrzak M, Stepinski J, Jemielity J, Kalek M, Bojarska E, Jankowska-Anyszka M, Davis RE, Darzynkiewicz E.; Kinetics of C elegans DcpS cap hydrolysis studied by fluorescence spectroscopy. Nucleos. Nucleot. Nucl. Acids 26, 1211-1215 (2007).
  12. Lewdorowicz M, Stepinski J, Kierzek R, Jemielity J, Zuberek J, Yoffe Y, Shapira M, Stolarski R, Darzynkiewicz E.; Synthesis of leishmania cap-4 intermediates, cap-2 and cap-3. Nucleos. Nucleot. Nucl. Acids 26, 1339-1348 (2007).
  13. Darzynkiewicz ZM, Bojarska E, Stepinski J, Jemielity J, Jankowska-Anyszka M, Davis RE, Darzynkiewicz E.; Affinity of dinucleotide cap analogues for human decapping scavenger (hdcps). Nucleos. Nucleot. Nucl. Acids 26, 1349-1352 (2007).
  14. Kalek M, Jemielity J, Darzynkiewicz ZM, Bojarska E, Stepinski J, Stolarski R, Davis RE, Darzynkiewicz E; Enzymatically Stable 5' mRNA Cap Analogs: Synthesis and Binding Studies with Human DcpS Decapping Enzyme. Bioorganic and Medicinal Chemistry 14, 3223-3230 (2006).
  15. Guranowski A, Starzyńska E, Pietrowska-Borek M, Jemielity J, Kowalska J, Darzynkiewicz E, Thompson MJ, Blackburn MG; Methylene analogues of adenosine-5'-tetraphosphate: their chemical synthesis and recognition by human and plant mononucleoside tetraphosphatases and dinucleoside tetraphosphatases. FEBS Journal 273, 829-838 (2006).
  16. Grudzien E, Kalek M., Jemielity J, Darzynkiewicz E, Rhoads RE; Differential inhibition of mRNA degradation pathways by novel cap analogs. Journal of Biological Chemistry 281, 1857-186 (2006).
  17. Westman B, Beeren L, Grudzien E, Stepinski J, Worch R, Zuberek J, Jemielity J, Stolarski R, Darzynkiewicz E, Rhoads RE, Preiss T; The antiviral drug ribavirin does not mimic the 7-methylguanosine moiety of the mRNA cap structure in vitro. RNA 11, 1505-1513 (2005).
  18. Kowalska J, Lewdorowicz M, Zuberek J, Bojarska E, Wojcik J, Cohen LS, Davis RE, Stepinski J, Stolarski R, Darzynkiewicz E, Jemielity J; Synthesis of mRNA cap analogues containing phosphorothioate moiety in 5',5'-triphosphate chain. Nucleos. Nucleot. Nucl. Acids, 24, 595-600 (2005).
  19. Jemielity J, Heinonen P, Lönnberg H, Darzynkiewicz E; Novel approach to solid phase chemical synthesis of oligonucleotide mRNA cap analogues, Nucleos. Nucleot. Nucl. Acids 24, 601-605 (2005).
  20. Kalek M, Jemielity J, Zuberek J, Grudzień E, Bojarska E, Cohen LS, Stepinski J, Stolarski R, Davis RE, Rhoads RE, Darzynkiewicz E; Synthesis and biochemical properties of novel mRNA 5' cap analogs resistant to enzymatic hydrolysis. Nucleos. Nucleot. Nucl. Acids 24, 615-621 (2005).
  21. Jemielity J, Pietrowska-Borek M, Starzynska E, Kowalska J, Stolarski R, Guranowski A, Darzynkiewicz E; Synthesis and Enzymatic Characterization of Methylene Analogs of Adenosine 5'-Tetraphosphate (p4A). Nucleos. Nucleot. Nucl. Acids 24, 589-593 (2005).
  22. Stepinski J, Zuberek J, Jemielity J, Kalek M, Stolarski R, Darzynkiewicz E; Novel Dinucleoside 5',5'-Triphosphate Cap Analogues. Synthesis and Affinity for Murine Translation Factor eIF4E. Nucleos. Nucleot. Nucl. Acids 24, 629-633 (2005).
  23. Kalek M, Jemielity J, Stepinski J, Stolarski R, Darzynkiewicz E; A Direct Method for the Synthesis of Nucleoside 5'-Methylenebis(phosphonate)s from Nucleosides. Tetrahedron Letters 46, 2417-2421 (2005).
  24. Lewdorowicz M, Yoffe Y, Zuberek J, Jemielity J, Stepinski J, Kierzek R, Stolarski R, Shapira M, Darzynkiewicz E; Chemical synthesis and binding activity of the trypanosomatid cap-4 structure. RNA 10, 1469-1478 (2004).
  25. Zuberek J, Jemielity J, Jablonowska A, Stepinski J, Dadlez M, Stolarski R, Darzynkiewicz E; Influence of electric charge variation at residues 209 and 159 on the interaction of eIF4E with the mRNA 5 ' terminus. Biochemistry 43, 5370-5379, (2004).
  26. Jemielity J, Fowler T, Zuberek J, Stepinski J, Lewdorowicz M, Niedzwiecka A, Stolarski R, Darzynkiewicz E, Rhoads RE. Novel "anti-reverse" cap analogs with superior translational properties. RNA 9, 1108-1122 (2003).
  27. Jemielity J, Stepinski J, Jaremko M, Haber D, Stolarski R, Rhoads RE, Darzynkiewicz E; Synthesis of novel mRNA 5 ' cap-analogues: Dinucleoside P-1, P-3-tri-, P-1, P-4-tetra-, and P-1, P-5-pentaphosphates. Nucleosides Nucleotides & Nucleic Acids 22, 691-694 (2003).
  28. Zuberek J, Jemielity J, Stepinski J, Lewdorowicz M, Niedzwiecka A, Haber D, Stolarski R, Rhoads RE, Darzynkiewicz E; Binding studies of eukaryotic initiation factor eIF4E with novel mRNA dinucleotide cap analogues. Nucleosides Nucleotides & Nucleic Acids 22, 1703-1706 (2003).
  29. Zuberek J, Jemielity J, Niedzwiecka A, Stepinski J, Wyslouch-Cieszynska A, Stolarski R, Darzynkiewicz E; Influence of the length of the phosphate chain in mRNA 5 ' cap analogues on their interaction with eukaryotic initiation factor 4E. Nucleosides Nucleotides & Nucleic Acids 22, 1707-1710 (2003)
  30. Kiraga-Motoszko K, Stepinski J, Niedzwiecka A, Jemielity J, Wszelaka-Rylik M, Stolarski R, Zielenkiewicz W, Darzynkiewicz E; Interaction between yeast eukaryotic initiation factor eIF4E and mRNA 5 ' cap analogues differs from that for murine eIF4E. Nucleosides Nucleotides & Nucleic Acids 22, 1711-1714 (2003)
  31. Augustyniak W., Suchecki P., Jemielity J., Kański R., Kańska M.; Synthesis of tritium labelled isotopomers of L-tyrosine. Journal of Labeled Compounds and Radiopharmaceuticals 45 (2002) 559-567.
  32. Jemielity J., Kański R., Kańska M.; Synthesis of tritium labeled [3S-3H]- and [3R-3H]-L- Phenylalanine. Journal of Labeled Compounds and Radiopharmaceuticals 44 (2001) 295-304.
  33. Augustyniak W., Bukowski J., Jemielity J., Kański R., Kańska M.; Ezymatic synthesis of carbon-14 labeled isotopomers of L- phenylalanine. Journal of Radioanalytical Nuclear Chemistry 247 (2000) 371-374.
  34. Lewandowicz A, Jemielity J, Kańska M, Zon J, Paneth P.; Tritium secondary kinetic isotope effect on phenylalanine ammonia-lyase catalyzed reaction. Archives of Biochemistry and Biophysics 370 (1999) 216-221.
  35. Jemielity J., Kańska M., Kański R.; Enzymatic Synthesis of [1-13C]- and [1-14]-L-Phenylalanine. Isotopes in Environmental and Health Studies 34 (1998) 335-339.
Participation in research projects:
Role of 5' mRNA and 5' U snRNA cap structures in regulation of gene expression