Professional experience:
| 1993 |
Msc. in physics and biophysics, Faculty of Physics, Warsaw University, Warsaw, Poland. |
| 1993-1999 |
Ph.D. Research, Department of Biophysics, Institute of Experimental Physics,
Warsaw University, Warsaw, Poland. |
| 1999 |
PhD in physics and biophysics, Department of Biophysics, Institute of Experimental
Physics, Warsaw University, Warsaw, Poland. |
| 2000 - |
Adjunct, Department of Biophysics, Institute of Experimental Physics, Warsaw University,
Warsaw, Poland. |
| 1996-2001 |
Practical research as a Visiting Scientist in the laboratory of Prof.
Wolfram Saenger, at Institute of Crystallography, Freie Universität, Berlin.
|
| 2003 |
Postdoctoral training at Dr.
Patricia Clark laboratory, University of Notre Dame, Notre Dame, Indiana, USA.
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Selected publications 1996-2005:
- B. Wielgus-Kutrowska, A. Bzowska, Kinetic properties of Cellulomonas sp.
purine nucleoside phosphorylase with typical and non-typical substrates: implications
for the reaction mechanism. Nucleosides, Nucleotides and Nucleic Acids 5-7, 471-476 (2005).
- A. Bzowska, K. Stepniak, M. Olasek, M. Długosz, B. Wielgus-Kutrowska, J. Antosiewicz,
A. Holy, G. Koellner, A. Stroh, G. Raszewski, T. Steiner and J. Frank, Attemps to
differentiate subunits of trimeric and hexameric purine nucleoside phosphorylase
by crystal structure and solution studies using purine bases, modified purine nucleosides,
acyclonucleosides and their phoshonate analogues. Chemistry of Nucleic Acid Components,
Collection Symposium Series, vol. 7, 133-142 (2005).
- A. Bzowska, G. Koellner, B. Wielgus-Kutrowska, A. Stroh, G. Raszewski, A. Holý,
T. Steiner, J. Frank, Crystal structure of calf spleen purine nucleoside phosphorylase
with two full trimers in the asymmetric unit: important implications for the mechanism
of catalysis. J. Mol. Biol. 342, 1015-1032 (2004).
- B. Wielgus-Kutrowska, A. Holy, J. Frank, G. Koellner and A. Bzowska, Interactions
of trimeric purine nucleoside phosphorylases with ground state analogues - calorimetric
and fluorimetric studies. Nucleosides, Nucleotides and Nucleic Acids 22, 1695-1698 (2003).
- B. Wielgus-Kutrowska, A. Bzowska, J. Tebbe, G. Koellner and D. Shugar,
Purine nucleoside phosphorylase from Cellulomonas sp.: physicochemical properties and
binding of substrates determined by ligand-dependent enhancement of enzyme intrinsic
fluorescence, and by protective effects of ligands on thermal inactivation of the enzyme.
Biochim. Biophys. Acta 1597, 320-334 (2002).
- G. Koellner, A. Bzowska, B. Wielgus-Kutrowska, M. Luić, T. Steiner, W. Saenger and
J. Stępiński, Open and closed conformation of the E. coli purine nucleoside phosphorylase
active center and implications for the catalytic mechanism. J. Mol. Biol. 315, 351-371
(2002).
- B. Wielgus-Kutrowska, J. Tebbe, J. Wierzchowski, D. Shugar, W. Saenger, G. Koellner
and A. Bzowska, Binding of substrates by purine nucleoside phosphorylase (PNP) from
Cellulomonas sp. - kinetic and spectrofluorimetric studies. Nucleosides and
Nucleotides 18, 871-872 (1999).
- J. Tebbe, A. Bzowska, B. Wielgus-Kutrowska, W. Schröder, Z. Kazimierczuk, D. Shugar,
W. Saenger, W. and G. Koellner, Crystal structures of purine nucleoside phosphorylase
(PNP) from Cellulomonas sp. and its implications of the molecular mechanism of trimeric
PNPs. J. Mol. Biol. 294, 1239-1255 (1999).
- A. Bzowska, L. Magnowska, B. Wielgus-Kutrowska and Z. Kazimierczuk, Synthesis of
2-chloro-6-aryloxy- and 2-chloro-6-alkoxyarylpurines and their properties in
purine nucleoside phosphorylase (PNP) system. Nucleos. And Nucleot.18 (4-5),
873-874 (1999).
- B. Wielgus-Kutrowska, J. Tebbe, W. Schröder, M. Luič, D. Shugar, W. Saenger,
G. Koellener and A. Bzowska, Cellulomonas sp. Purine Nucleoside Phosphorylase (PNP):
Comparison with Human and E. Coli Enzymes. Adv. Exp. Med. Biol. 431, 259-264,
Plenum Publishing Corp., New York (1998) .
- A. Bzowska, J. Tebbe, M. Luič, B. Wielgus-Kutrowska, W. Schröder, D. Shugar,
W. Saenger, and G. Koellener, Crystallization and preliminary studies of purine nucleoside
phosphorylase from Cellulomonas sp. Acta Cryst. D54, 1061-1063 (1998).
- B. Wielgus-Kutrowska, E. Kulikowska, J. Wierzchowski,A. Bzowska and D. Shugar,
Nicotinamide riboside - an unusual, non-typical substrate of purified purine nucleoside
phosphorylases. Eur. J. Biochem. 243, 408-414 (1997).
- J. Wierzchowski, B. Wielgus-Kutrowska and D. Shugar, Fluorescence emission of
8-azapurines and their nucleosides, and application to the kinetic reverse synthetic
reaction of purine nucleoside phosphorylase. Biochim. Biophys. Acta 1290, 9-17 (1996).
- J. Tebbe, B. Wielgus-Kutrowska, W. Schröder, M. Luič, D. Shugar, W. Saenger,
G. Koellener and A. Bzowska, Purine nucleoside phosphorylase (PNP) from Cellulomonas sp.,
a third class of PNP different from both "low-molecular weight" mammalian and
"high-molecular weight" bacterial PNPs. Miami, Nature Biotechnology,
Short Reports, Vol. 8, p. 90, Oxford University Press (1996).
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