| Name: Dr. hab. Michał Dadlez
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Address: Department of Biophysics,
Institute of Experimental Physics, Warsaw University,
Zwirki i Wigury 93, 02-089 Warsaw, Poland
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| Phone: +48 22 6597072
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| E-mail:
michald@biogeo.uw.edu.pl
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Research interests:
protein folding, aggregation, complexes; proteomics; mass spectrometry of non-covalent interactions.
Group interests involve proteomic and structural protein studies with special focus on molecular
complexes involved in the functioning of the neural system. We are using diverse biophysical methods
to characterise these complexes. We develop new approaches to use mass spectrometry of non-covalent
complexes to gain insight into weakly associating molecular entities - peptides and proteins. We are
also involved in several proteomics projects, being responsible for identification of proteins of
interest and characterisation of their post translational modifications. This activity is illustrated
on IBB PAS, Mass Spectrometry and Proteomics Lab page:
http://poczta.ibb.waw.pl/~proteom/
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Professional experience:
| 1975 - 1980 |
Graduate studies, Optoelectronics, Department of Electronics, Warsaw Technical University, Warsaw, Poland.
(Oct., 1980) M. Sc. Thesis "Possibility of radiation enhancement in thin, dielectric, neodymium doped lasers".
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| 1983 - 1991 |
Postgraduate studies. Institute of Biochemistry and Biophysics, Polish Academy of Sciences.
Work on peptide conformation using spectroscopic methods, like circular dichroism, luminescence, nuclear magnetic resonance.
(14th, Jan. 1992) Ph. D. Thesis: "Interactions stabilizing alpha helical conformation in short peptides". Supervisor Prof. Andrzej Bierzynski.
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| 1992 - 1994 |
Postdoctoral training. Supervisor Prof. P.S. Kim.; Whitehead Institute for Biomedical Research, MIT, HHMI, Cambridge, USA. Work on early folding events in proteins by studying the kinetics of the disulfide formation in bovine pancreatic trypsin inhibitor (BPTI). |
| 1995 - |
1995- IBB PAS, Research on protein folding, aggregation and protein-protein interactions. |
| 1999 |
Doctor habilitatus, IBB PAS, "The initiation of protein folding" |
| 2000 - |
2000- Leader of Mass Spectrometry group, IBB PAS, work on proteomic protein identification, post-translational modification detection, non-covalent interactions of proteins. |
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Selected publications:
- Davezac, N., Tondelier, D., Lipecka, J., Demaugre, F., Debski, J., Dadlez, M., Schrattenholtz, A., Cahill, M. & Edelman, A. (2003) Expression of Keratin8/18 regulates the biosynthetic pathway of DF508CFTR. Nature Cell Biology, submitted Apr'2003
- Dominski, Z., Yang, X., Kaygun, H., Dadlez, M. & Marzluff, W.F. (2003) Identification of a 3'exonuclease specifically interacting with the 3' end of histone mRNA, Mol. Cell submitted Mar'2003
- Zuberek, J., Wyslouch-Cieszynska, A., Niedzwiecka, A., Dadlez, M., Stepinski, J., Augustyniak, W., Gingras, A.-C., Lonnberg, H., Burley, S.K., Sonenberg, N., Stolarski, R., and Darzynkiewicz, E. (2003) Phosphorylation of eIF4E reduces its ability of binding cap analogs by electrostatic repulsion; adoption of IPL strategy for obtaining phosphorylated form of protein. RNA 9, 52-61
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| Participation in research projects: |
| EU project QLG2-CT2001-01335; "Proteomics in cystic fibrosis studies", coordinator: A. Edelman, Institut Necker, Paris, wykonawca.
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KBN grant zamawiany "Zastosowanie proteomiki w badaniach biologicznych i medycznych" - wykonawca
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KBN grant 6 P04A 004 20 "Oddziaływania stabilizujące strukturę oligomerów peptydów Abeta związanych z chorobą Alzheimera" - kierownik.
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| Inicjacja translacji: biofizyka oddziaływań białek i mRNA
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Research
