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Warsaw University Faculty of Physics
WERSJA POLSKA  Research    People    Education
Name: dr Anna Nied¼wiecka
Address: Department of Biophysics, Institute of Experimental Physics, Warsaw University,
Zwirki i Wigury 93, 02-089 Warsaw, Poland
Phone: +48 22 55 40 789
E-mail: ania@biogeo.uw.edu.pl
and
Address: Institute of Physics, Polish Academy of Sciences
Al. Lotników 32/46, 02-668 Warsaw, Poland
Phone: +48 22 843-66-01 int. 3516
E-mail: annan@ifpan.edu.pl
Research interests:
Selected publications:
  1. A. Nied¼wiecka, E. Dar¿ynkiewicz, R.Stolarski (2004): Thermodynamics of mRNA 5' Cap Binding by Eukaryotic Translation Initiation Factor eIF4E, Biochemistry, 43, 13305-13317. 
  2. J. Jemielity, T. Fowler, J. Zuberek, J. Stepinski, M. Lewdorowicz, A. Niedzwiecka, R. Stolarski, E. Darzynkiewicz, and R. E. Rhoads (2003): Novel 'anti-reverse' cap analogues with superior translational properties, RNA, 9, 1108-1122;
  3. A. Nied¼wiecka, J. Stêpiñski, L. Balaspiri, E. Dar¿ynkiewicz and R. Stolarski (2003): Thermodynamics of 7-methylguanosine cation stacking with tryptophan upon mRNA 5' cap binding to translation factor eIF4E, Nucleos., Nucleot. & Nucl. Acids, 22, 1557-1561;
  4. K. Kiraga-Motoszko, J. Stêpiñski, A. Nied¼wiecka, J. Jemielity, M. Wszelaka-Rylik, R. Stolarski, W. Zielenkiewicz and E. Dar¿ynkiewicz (2003): Interaction between yeast eukaryotic initiation factor eIF4E and mRNA 5' cap analogues differs from that for murine eIF4E, Nucleos., Nucleot. & Nucl. Acids, 22, 1711-1714;
  5. J. ¯uberek, J. Jemielity, J. Stêpiñski, A. Nied¼wiecka, A. Wys³ouch-Cieszyñska, R. Stolarski, E. Dar¿ynkiewicz (2003): Influence of the length of phosphate chain in mRNA 5' cap analogues on their interaction with eukaryotic initiation factor 4E, Nucleos., Nucleot. & Nucl. Acids, 22, 1707-1710;
  6. J. ¯uberek, J. Jemielity, J. Stêpiñski, M. Lewdorowicz, A. Nied¼wiecka, D. Haber, F. Tolvert, R. Stolarski, R.E. Rhoads, E. Dar¿ynkiewicz (2003): Binding studies of eukaryotic initiation factor eIF4E with novel mRNA dinucleotide cap analogues, Nucleos., Nucleot. & Nucl. Acids, 22, 1703-1706;
  7. J. ¯uberek, A. Wyslouch-Cieszyñska, A. Nied¼wiecka, M. Dadlez, J. Stêpiñski, W. Augustyniak, A.-C. Gingras, Z. Zhang, S. K. Burley, N. Sonenberg, R. Stolarski and E. Dar¿ynkiewicz (2003) Phosphorylation of eIF4E attenuates its interaction with mRNA cap analogs by electrostatic repulsion; Intein-Mediated Protein Ligation strategy to obtain phosphorylated protein, RNA, 9, 52-61.
  8. Nied¼wiecka,A., J.Stêpiñski, E.Dar¿ynkiewicz, N.Sonenberg, and R.Stolarski. (2002). Positive heat capacity change upon specific binding of translation factor eIF4E to mRNA 5' cap. Biochemistry, 41, 12140-12148.
  9. Nied¼wiecka,A., J.Marcotrigiano, J.Stêpiñski, M.Jankowska-Anyszka, A.Wys³ouch-Cieszynska, M.Dadlez, A.C.Gingras, P.Mak, E.Dar¿ynkiewicz, N.Sonenberg, S.K.Burley, and R.Stolarski. (2002). Biophysical studies of eIF4E cap-binding protein: recognition of mRNA 5' cap structure and synthetic fragments of eIF4G and 4E-BP1 proteins. J. Mol. Biol., 319, 615-635.
  10. Gingras,A.C., B.Raught, S.P.Gygi, A.Nied¼wiecka, M.Miron, S.K.Burley, R.D.Polakiewicz, A.Wys³ouch-Cieszynska, R.Aebersold, and N.Sonenberg. (2001). Hierarchical phosphorylation of the translation inhibitor 4E-BP1. Gene Dev., 15, 2852-2864.
  11. B³achut-Okrasinska,E., E.Bojarska, A.Nied¼wiecka, L.Chlebicka, E.Dar¿ynkiewicz, R.Stolarski, J.Stêpiñski, and J.M.Antosiewicz. (2000). Stopped-flow and Brownian dynamics studies of electrostatic effects in the kinetics of binding of 7-methyl-GpppG to the protein eIF4E. Eur. Biophys. J., 29, 487-498.
  12. Wieczorek,Z., A.Nied¼wiecka-Korna¶, L.Chlebicka, M.Jankowska, K.Kiraga, J.Stêpiñski, M.Dadlez, R.Drabent, E.Dar¿ynkiewicz, and R.Stolarski. (1999). Fluorescence studies on association of human translation initiation factor eIF4E with mRNA cap-analogues. Z. Naturforsch. [C], 54, 278-284.
  13. Nied¼wiecka-Korna¶,A., R.Przedmojski, L.Balaspiri, Z.Wieczorek, J.Stêpiñski, M.Jankowska, H.Lönnberg, E.Dar¿ynkiewicz, and R.Stolarski. (1999). Studies on association of mRNA cap-analogues with a synthetic dodecapeptide DGIEPMWEDEKN. Nucleosides & Nucleotides, 18, 1105-1106. 
  14. Nied¼wiecka-Korna¶,A., B. Kierdaszuk, R. Stolarski and D. Shugar. (1998) Tautomerism, acid-base properties and conformation of methylated analogues of the promutagenic N4-hydroxycytosine, Biophysical Chemistry, 71, 87-98.
  15. Orzeszko,A., J. K. Maurin, A. Nied¼wiecka-Korna¶ and Z. Kazimierczuk (1998) The novel product of cathodic reduction of phtalimide anion, Tetrahedron, 54, 7517-7524.
  16. Orzeszko,A., A. Nied¼wiecka-Korna¶, R. Stolarski and Z. Kazimierczuk (1998) Synthesis and conformation of nucleoside 5'- thiosulfates, Z. Naturforsch. [B], 53 1191-1196.
Participation in research projects:
Translation initiation and intracellular transport: biophysics of protein and RNA interactions
Role of 5' mRNA and 5' U snRNA cap structures in regulation of gene expression