Molecular genetics

Plan of the lecture:

1. Strukture and function of deoxyribonucleic acids (DNA)
   1. Biological role and types of of double helix
   2. Supercoiling
   3. Chromosome structure
   4. Methods of DNA investigations
2. Transcription
   1. DNA sequence organization and role of DNA as a matrix
   2. Transcription complex and regulation of the transcription process
   3. Transcription and nucleosomes
3. Structure and function of ribonucleic acids (RNA)
   1. RNA maturation: splicing, capping) i and polyadenylation
   2. Intracellular transport of RNA
4. Protein biosynthesis (translation)
5. Molecular mechanisms of interation between proteins and nucleic acids in gene expression
6. Genetic engineering
   1. Construction of the gene for recombination
   2. Introducing the recombinant gene into prokaryotic and eukaryotic cells
   3. Analysis of the recombinant cells
   4. Gene and genome sequencing
7. Practical aspects of molecular genetics
   1. Molecular medicine
   2. Genetic modifications of microorganisms, plants and animals in a control manner

Literature:

1. T. A. Brown: Genomy. PWN, Warszawa 2001
2. L. Stryer: Biochemia
3. B. Alberts i wsp.: Podstawy biologii komórki. Wprowadzenie do biologii molekularnej. PWN, Warszawa 1999.
4. Genetyka molekularna, pod red. Piotra Węgleńskiego