Biochemistry Laboratory

Topics:

1. Determination of protein concentration in a solution.
2. Determination of the kinetic parameters (V_max and K_m) in enzymatic reactions.
3. Polyacrylamide gel electrophoresis analysis of proteins under denaturating conditions.
4. Isolation of DNA from calf thymus gland.
5. Fractionation of rat liver according to Schneider method and quantitative determination of nucleic acids in it.

Literature:

1. "Ćwiczenia z Biochemii" red. L. Kłyszejko-Stefanowicz, PWN, Warszawa 1999
2. L. Stryer "Biochemia", PWN, Warszawa 1997
3. J. Walory, M. Pilarek, M. Kalinowska, H. Jaworska-Deptuch "Biochemia, ćwiczenia laboratoryjne" WPW, Warszawa 2003